1x taqman gene expression 19 assay probes (Thermo Fisher)
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1x taqman gene expression 19 assay probes
1x Taqman Gene Expression 19 Assay Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taqman+gene+expression+probes/SUCROSE+EP%2FBP%2FNF+12KG/pm41546116-255-38-45
Average 98 stars, based on 1 article reviews
1x Taqman Gene Expression 19 Assay Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taqman+gene+expression+probes/SUCROSE+EP%2FBP%2FNF+12KG/pm41546116-255-38-45
Average 98 stars, based on 1 article reviews
1x taqman gene expression 19 assay probes - by Bioz Stars,
2026-09
98/100 stars
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Gene Expression:Article Title: In Vivo Contribution of Cyp24a1 Promoter Vitamin D Response Elements Article Snippet: One μg of isolated total RNA was DNase treated, reverse transcribed using the High-Capacity cDNA Kit (Applied Biosystems), and then diluted to 100 μL with RNase/DNase-free water. qPCR was performed using primers specific to a select set of differentially expressed genes by TaqMan analyses. .. Article Title: The diazirine-mediated photo-crosslinking of collagen improves biomaterial mechanical properties and cellular interactions. Article Snippet: In tissue engineering, crosslinking with carbodiimides such as EDC is omnipresent to improve the mechanical properties of biomaterials.. However, in collagen biomaterials, EDC reacts with glutamate or aspartate residues, inactivating the binding sites for cellular receptors and rendering collagen inert to many cell types.. In this work, we have developed a crosslinking method that ameliorates the rigidity, stability, and degradation rate of collagen biomaterials, whilst retaining key interactions between cells and the native collagen sequence. Article Title: Transcriptomic Changes During the Replicative Senescence of Human Articular Chondrocytes Article Snippet: Total RNA was isolated from samples snap-frozen in liquid nitrogen and homogenized in an Eppendorf tube using 3 mm Tungsten carbide beads and a TissueLyser II system (Qiagen, Germantown, MD, USA) set to 4 min at 30 Hz; 1 μg of cDNA was synthesized using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Waltham, MA, USA). .. PCR experiments were performed using 20 ng of cDNA per reaction, qRT-PCR Brilliant III ultra-fast master mix kit (Agilent, Santa Clara, CA, USA), and Article Title: Phlebotomy-induced anemia reduces oxygen-induced retinopathy severity and dampens retinal developmental transcriptomic pathways in rats. Article Snippet: BACKGROUND: Phlebotomy-induced-anemia (PIA), which induces tissue hypoxia and angiogenesis, occurs universally among infants at risk for severe retinopathy of prematurity (ROP).. We hypothesized that PIA exacerbates pathologic retinal neovascularization in ROP.. METHODS: We induced PIA to a hematocrit of 18% among rats undergoing the established 50/10 oxygen-induced retinopathy (OIR) model. Article Title: In vivo contribution of Cyp24a1 promoter vitamin D response elements Article Snippet: 1 μg of isolated total RNA was DNase treated, reverse transcribed using the High-Capacity cDNA Kit (Applied Biosystems), and then diluted to 100 μL with RNase/DNase free water. qPCR was performed using primers specific to a select set of differentially expressed genes by TaqMan analyses. .. Article Title: Arginase-1-specific T cells target and modulate tumor-associated macrophages Article Snippet: mRNA was isolated using the RNeasy Plus mini kit (Quiagen, cat. 74136) following the manufacturer’s guidelines. cDNA was synthesized from 1 μg RNA using the Maxima first strand cDNA synthesis kit for RT-qPCR (Thermo Scientific, cat. K1671). .. Gene expression was assessed by RT-qPCR using TaqMan Fast advanced master mix (Applied Biosystems, cat. 4444963) and Article Title: α7 nicotinic acetylcholine receptor agonist attenuates allergen-induced immediate nasal response in murine model of allergic rhinitis Article Snippet: Reverse transcription was performed using SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) with random primers after total RNA extraction from the nasal-associated lymphoid tissues of the Th2 cell-transplanted model. .. The quantitative RT-PCR for Tcrb of DO11.10 T cells (DO11.10-Tcrb), Il4 (Mm00445259_m1), Epx (Mm00514768_m1), and Enpp3 (Mm01193723_m1) was performed using the Article Title: Spatial detection and consequences of nonrenal calcitriol production as assessed by targeted mass spectrometry imaging. Article Snippet: 1 μg of isolated total RNA was DNase treated, reverse transcribed using the High-Capacity cDNA Kit (Applied Biosystems), and then diluted to 100 μL with RNase/DNase free water. qPCR was performed using primers specific to a select set of differentially expressed genes by TaqMan analyses. .. Quantitative RT-PCR:Article Title: The diazirine-mediated photo-crosslinking of collagen improves biomaterial mechanical properties and cellular interactions. Article Snippet: In tissue engineering, crosslinking with carbodiimides such as EDC is omnipresent to improve the mechanical properties of biomaterials.. However, in collagen biomaterials, EDC reacts with glutamate or aspartate residues, inactivating the binding sites for cellular receptors and rendering collagen inert to many cell types.. In this work, we have developed a crosslinking method that ameliorates the rigidity, stability, and degradation rate of collagen biomaterials, whilst retaining key interactions between cells and the native collagen sequence. Article Title: Transcriptomic Changes During the Replicative Senescence of Human Articular Chondrocytes Article Snippet: Total RNA was isolated from samples snap-frozen in liquid nitrogen and homogenized in an Eppendorf tube using 3 mm Tungsten carbide beads and a TissueLyser II system (Qiagen, Germantown, MD, USA) set to 4 min at 30 Hz; 1 μg of cDNA was synthesized using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Waltham, MA, USA). .. PCR experiments were performed using 20 ng of cDNA per reaction, qRT-PCR Brilliant III ultra-fast master mix kit (Agilent, Santa Clara, CA, USA), and Article Title: Arginase-1-specific T cells target and modulate tumor-associated macrophages Article Snippet: mRNA was isolated using the RNeasy Plus mini kit (Quiagen, cat. 74136) following the manufacturer’s guidelines. cDNA was synthesized from 1 μg RNA using the Maxima first strand cDNA synthesis kit for RT-qPCR (Thermo Scientific, cat. K1671). .. Gene expression was assessed by RT-qPCR using TaqMan Fast advanced master mix (Applied Biosystems, cat. 4444963) and Article Title: α7 nicotinic acetylcholine receptor agonist attenuates allergen-induced immediate nasal response in murine model of allergic rhinitis Article Snippet: Reverse transcription was performed using SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) with random primers after total RNA extraction from the nasal-associated lymphoid tissues of the Th2 cell-transplanted model. .. The quantitative RT-PCR for Tcrb of DO11.10 T cells (DO11.10-Tcrb), Il4 (Mm00445259_m1), Epx (Mm00514768_m1), and Enpp3 (Mm01193723_m1) was performed using the Polymerase Chain Reaction:Article Title: Transcriptomic Changes During the Replicative Senescence of Human Articular Chondrocytes Article Snippet: Total RNA was isolated from samples snap-frozen in liquid nitrogen and homogenized in an Eppendorf tube using 3 mm Tungsten carbide beads and a TissueLyser II system (Qiagen, Germantown, MD, USA) set to 4 min at 30 Hz; 1 μg of cDNA was synthesized using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Waltham, MA, USA). .. PCR experiments were performed using 20 ng of cDNA per reaction, qRT-PCR Brilliant III ultra-fast master mix kit (Agilent, Santa Clara, CA, USA), and Synthesized:Article Title: Phlebotomy-induced anemia reduces oxygen-induced retinopathy severity and dampens retinal developmental transcriptomic pathways in rats. Article Snippet: BACKGROUND: Phlebotomy-induced-anemia (PIA), which induces tissue hypoxia and angiogenesis, occurs universally among infants at risk for severe retinopathy of prematurity (ROP).. We hypothesized that PIA exacerbates pathologic retinal neovascularization in ROP.. METHODS: We induced PIA to a hematocrit of 18% among rats undergoing the established 50/10 oxygen-induced retinopathy (OIR) model. Real-time Polymerase Chain Reaction:Article Title: Phlebotomy-induced anemia reduces oxygen-induced retinopathy severity and dampens retinal developmental transcriptomic pathways in rats. Article Snippet: BACKGROUND: Phlebotomy-induced-anemia (PIA), which induces tissue hypoxia and angiogenesis, occurs universally among infants at risk for severe retinopathy of prematurity (ROP).. We hypothesized that PIA exacerbates pathologic retinal neovascularization in ROP.. METHODS: We induced PIA to a hematocrit of 18% among rats undergoing the established 50/10 oxygen-induced retinopathy (OIR) model. Article Title: α7 nicotinic acetylcholine receptor agonist attenuates allergen-induced immediate nasal response in murine model of allergic rhinitis Article Snippet: Reverse transcription was performed using SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) with random primers after total RNA extraction from the nasal-associated lymphoid tissues of the Th2 cell-transplanted model. .. The quantitative RT-PCR for Tcrb of DO11.10 T cells (DO11.10-Tcrb), Il4 (Mm00445259_m1), Epx (Mm00514768_m1), and Enpp3 (Mm01193723_m1) was performed using the |